abstract：:A bioanalytical method using automated sample transferring, automated solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS-MS) was developed for morphine (MOR), and its metabolites morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G) in human plasma. Samples of 0.25 ml were t...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Shou WZ,Pelzer M,Addison T,Jiang X,Naidong W

更新日期：2002-01-01 00:00:00

abstract：:The nonreceptor tyrosine kinase FynB is known to be required in the induction of long-term potentiation (LTP), a cellular mechanism for learning and memory. Ligands of the FynB SH2 domain as a possible FynB activator are, thus, of great interest. In this study, a solid-phase ligand binding assay was established to mee...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kim HJ,Lee HH,Yoo HD,Hwa Lee J,Hong ST

更新日期：2002-01-01 00:00:00

abstract：:An on-line liquid chromatography-immunochemical detection (LC-ICD) system for the quantification of cytokines in cell extracts has been developed using a post-column continuous-flow reaction detection system using fluorescence labelled antibodies. Cytokines eluting from the micro-HPLC column react with antibodies to f...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Schenk T,Irth H,Marko-Varga G,Edholm LE,Tjaden UR,van der Greef J

更新日期：2001-12-01 00:00:00

abstract：:Micellar electrokinetic chromatography (nMEKC) method was applied to the determination of the partition behaviour between water and micelle for a group of antibiotics using sodium dodecyl sulphate (SDS) as an anionic model surfactant. In the method, the running buffer contains both the micelles and the drug, and the i...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Mrestani Y,Marestani Z,Neubert RH

更新日期：2001-12-01 00:00:00

abstract：:Praziquantel, a broad spectrum anthelmintic drug, is extensively metabolized in the liver, yielding mainly monohydroxylated and dihydroxylated phase-I metabolites. However, the exact chemical structure of most metabolites is still unknown. One of these unidentified phase-I metabolites was isolated from human urine by ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Schepmann D,Blaschke G

更新日期：2001-12-01 00:00:00

abstract：:This research presents the results obtained from analysis by thin layer chromatography (TLC) of some free amino acids from sanguine plasma samples in the different degree progress in maladies: diabetes, renal syndrome and hepatic cirrhosis. The chromatograms were evaluated with a Shimadzu CS-9000 dual-wavelength flyin...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Simion G,Liana G,Letiţia G

更新日期：2001-11-01 00:00:00

abstract：:The controlled adsorptive accumulation of Zn(II)-terfenadine complex (1:1) onto a hanging mercury drop electrode (HMDE) provides the basis for determination of the antihistamine drug terfenadine by differential pulse cathodic adsorptive stripping voltammetry. The adsorbed Zn(II)-terfenadine complex (1:1) at the HMDE d...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ghoneim M,Issa R,Tawfik A

更新日期：2001-11-01 00:00:00

abstract：:A numerical method, based on the use of spectrophotometric data coupled to PLS-1 multivariate calibration, is reported for the simultaneous determination of furosemide and amiloride hydrochloride in synthetic samples and commercial tablets. The method was applied in the concentration ranges of 8.0-13.0 mg l(-1) for fu...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Ferraro MC,Castellano PM,Kaufman TS

更新日期：2001-10-01 00:00:00

abstract：:The current study assesses the effect of extending column length during gradient packed column sub/supercritical fluid chromatography (PCSFC) experiments on the detection of known and unknown impurities in a drug substance sample. Quantitative drug substance impurity profiles were generated and compared using multiple...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Roston DA,Ahmed S,Williams D,Catalano T

更新日期：2001-10-01 00:00:00

abstract：:A method is described for the high performance liquid chromatographic (HPLC) determination of phenylpropanolamine (PPA) based on precolumn derivatization with 4-dimethylaminobenzaldehyde (DAB) and elution from phenomenex C-18 column with methanol-water and detection by spectrophotometry at 418 nm. Linear calibration w...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rind FM,Khuhawar MY,Rajper AD

更新日期：2001-09-01 00:00:00

abstract：:Combined infrared--mass spectra (IR--MS) have been used to examine a small data set of synthetic substances in order to elucidate whether a combination of spectral descriptors yield better classification and similarity predictions than their corresponding individual spectral descriptors. To eliminate differences in va...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Schoonjans V,Massart DL

更新日期：2001-09-01 00:00:00

abstract：:A rugged LC-MS/MS method was developed to quantify reduced and oxidized glutathione (GSH and GSSG, respectively) in rat hepatocytes. In addition, GSH conjugates can be detected, characterized and measured in the same analysis. Samples were treated with acetonitrile and iodoacetic acid to precipitate proteins and trap ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Loughlin AF,Skiles GL,Alberts DW,Schaefer WH

更新日期：2001-08-01 00:00:00

abstract：:Mobile phase optimization and reversed-phase column characteristics were used to separate photodegradation products from the parent compound, 24-cyclopropyl-9-,10-secochola-5,7,10(19),22-tetraene-1alpha,3beta,24-triol (calcipotriol). Separation between calcipotriol and its degradation products was obtained with an ace...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cirunay JJ,Vander Heyden Y,Plaizier-Vercammen J

更新日期：2001-08-01 00:00:00

abstract：:A simple, rapid and reproducible method for identification and quantification of iodopropynyl butylcarbamate (IPBC) in different cosmetic formulations is presented. The determination was carried out using a high-performance liquid chromatography (HPLC) procedure on a reversed phase column coupled to a single quadrupol...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Frauen M,Steinhart H,Rapp C,Hintze U

更新日期：2001-07-01 00:00:00

abstract：:The extraction of diclofenac from spiked aqueous and plasma samples by liquid-liquid extraction (LLE) and solid phase extraction (SPE) methods is compared. The SPE methodology utilised a hydrophilic lipophilic balanced (HLB) copolymer as the extraction phase. Using a literature HPLC method, a calibration curve for dic...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Millership JS,Hare LG,Farry M,Collier PS,McElnay JC,Shields MD,Carson DJ

更新日期：2001-07-01 00:00:00

abstract：:Capillary electrophoresis (CE) was applied to the study of several inhibitors of the angiotensin-converting enzyme. Separation of the compounds was performed by means of two phosphate buffers (each 100 mM) at pH 7.0 and 6.25, respectively [S. Hillaert, W. Van den Bossche, J. Chromatogr. A, 895 (2000) 33-42.]. Due to t...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hillaert S,Van den Bossche W

更新日期：2001-07-01 00:00:00

abstract：:A simple, rapid, reproducible and stability indicating high performance thin-layer chromatographic method for the analysis of Nimesulide both the bulk drug and from pharmaceutical formulations is reported. The mobile phase selected consists of Cyclohexane-Ethylacetate [60:40, v/v]. It gave compact spots both for Nimes...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Patravale VB,D'Souza S,Narkar Y

更新日期：2001-06-01 00:00:00

abstract：:A simple high-performance liquid chromatographic assay with using UV detection (266 nm) was developed to determine a novel gastroprokinetic agent, Z-338 in dog plasma. The extraction procedure using solid-phase extraction with a Isolute SCX column produces extremely clean eluates and a high recovery. Intra- and inter-...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Furuta S,Miyahara H,Sugimoto T,Sano H

更新日期：2001-06-01 00:00:00

abstract：:A circular dichroism (CD) detector in series with a UV detector was used with reversed-phase gradient HPLC to do the chiral analysis of unresolved enantiomers of a single stereoisomer drug substance in extracts of clinical plasma samples. The CD/UV peak area ratio of the unresolved enantiomers in the plasma extracts w...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Williams RC,Edwards JF,Joshi AS,Aubry AF

更新日期：2001-06-01 00:00:00

abstract：:A direct enantioselective high-performance liquid chromatography was employed successfully for determination of the enantiomeric purity of levamisole. The elaborated method used S-naphthylethylcarbamoylated beta-cyclodextrin stationary phase in reversed-phase mode. The optimized mobile phase composition was acetonitri...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Dolezalová M,Tkaczyková M

更新日期：2001-06-01 00:00:00

abstract：:A simple and sensitive liquid chromatographic method with electrochemical (EC) detection is described for the quantitative determination of amoxycillin and clavulanic acid in pharmaceutical dosage forms. Sample components were separated by a reversed phase C18 column, using a mixture of methanol-phosphate buffer (pH 3...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Aghazadeh A,Kazemifard G

更新日期：2001-05-01 00:00:00

abstract：:Highly selective non-covalent clenbuterol (CL) imprinted polymers were prepared using methacrylic acid as monomer and ethylene glycol dimethacrylate as cross-linking agent. HPLC experiments with columns packed with this material showed that CL are selectively recognised with respect to all other adrenergic substances ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Masci G,Casati G,Crescenzi V

更新日期：2001-05-01 00:00:00

abstract：:A simple reversed-phase high-performance liquid chromatography method was developed for the determination of s-carboxymethyl-l-cysteine in syrup preparations. The experiments were performed without specific sample pre-treatment. The LC conditions used were acetonitrile-10 mM sodium dihydrogenphosphate buffer, pH 2.0 (...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Suntornsuk L

更新日期：2001-04-01 00:00:00

abstract：:A simple, precise and accurate high performance liquid chromatography (HPLC) method was developed for the simultaneous estimation of metformin with gliclazide and glipizide present in multicomponent dosage forms. The method was carried out on Inertsil C(18) column. A mobile phase composed of acetonitrile-water contain...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Vasudevan M,Ravi J,Ravisankar S,Suresh B

更新日期：2001-04-01 00:00:00

abstract：:Total plasma homocysteine (tHcy) in children may be an useful biochemical marker for genetic risk of premature cardiovascular disease. We reported a rapid, isocratic HPLC method able to process very small amount of newborn plasma samples. A blood sample from heel capillary circulation was collected, using a heparinize...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Bartesaghi S,Accinni R,De Leo G,Cursano CF,Campolo J,Galluzzo C,Vegezzi PG,Parodi O

更新日期：2001-03-01 00:00:00

abstract：:A reversed-phase high-performance liquid chromatographic (RP-HPLC) separation method with UV spectrophotometric detection has been developed for the determination of major components in leaves and caulomas of Schisandra chinensis Baill. The flavonols (quercetin and kaempferol) and (E)-cinnamic acid were analysed after...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Sladkovský R,Solich P,Opletal L

更新日期：2001-03-01 00:00:00

abstract：:The new approach to drug development, especially for cardiovascular and brain diseases, brought to synthesis of new lipophillic derivatives of strong calcium chelator BAPTA - DP-b99 and DP-109. Due to their chelating ability, these compounds require metal-free stationary phases, and their high hydrophobicity resulted ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kalendarev T,Zupkovitz G,Ioffe V

更新日期：2001-03-01 00:00:00

abstract：:The inclusion complex formation between Metoprolol tartarata (MeT) and beta-cyclodextrin (beta-CD) has been investigated using hyperchromic shift at lambda(max) 274.4 nm of MeT. Different parameters such as stirring time, solvent composition (aqueous and aqueous/methanol solutions with methanol content up to 50%), pH ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Radulovic DM,Karljikovic-Rajic KD,Lucic BM,Vujic ZB

更新日期：2001-03-01 00:00:00

abstract：:High throughput transducers using metal cluster resonance technology are based on surface-enhancement of metal cluster light absorption. These devices can be used for detection of biorecognitive binding, as well as structural changes of nucleic acids, proteins or any other polymer. The optical property for the analyti...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Mayer C,Stich N,Palkovits R,Bauer G,Pittner F,Schalkhammer T

更新日期：2001-03-01 00:00:00

abstract：:A simple high-performance liquid chromatographic method was developed for the determination of omeprazole in human plasma. Omeprazole and the internal standard, chloramphenicol, were extracted from alkalinized plasma samples using dichloromethane. The mobile phase was 0.05 M Na2HPO4-ACN (65:35, v/v) adjusted to pH 6.5...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Yuen KH,Choy WP,Tan HY,Wong JW,Yap SP

更新日期：2001-02-01 00:00:00

abstract：:Micellar affinity capillary electrophoresis (MACE) was introduced to evaluate the affinity of various kinds of drugs as benzoic acid, salicylic acid, trinitrophenol, p-hydroxybenzoic acid and o-acetylsalicylic acid. Non-ionic micelles as Brij 35 (polyethylenglycol dodecylether), Tagat (polyoxyethylene (20) glycerol mo...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Mrestani Y,Neubert RH

更新日期：2001-02-01 00:00:00

abstract：:A capillary electrophoresis method using cyclodextrins as the chiral selectors was developed for the determination of carvedilol enantiomers in serum. Several types of cyclodextrins were evaluated. The effect of cyclodextrin concentration on enantiomer resolution was investigated. Best results were obtained using 10 m...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Clohs L,McErlane KM

更新日期：2001-02-01 00:00:00

abstract：:Glimepiride (Amaryl), which is a new oral antidiabetic drug in the sulfonylurea class, was analysed by using second order derivative UV spectrophotometry. The quantification of glimepiride in dimethylformamide was performed in the wavelength range of 245-290 nm at N = 6, ?lambda = 21. The second order derivative spect...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Altinöz S,Tekeli D

更新日期：2001-01-01 00:00:00

abstract：:The diuretic drug indapamide has been characterized voltammetrically at carbon paste electrodes by means of cyclic and differential pulse voltammetry. An adsorptive stripping method at carbon paste electrode modified with castor oil for trace determination of indapamide was described. A study of the variation of the p...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Radi A

更新日期：2001-01-01 00:00:00

abstract：:The hydrophobicity of human recombinant interleukin 11 (rhIL-11) with an oxidized Met58 residue is nearly identical to the hydrophobicity of native rhIL-11. Consequently, separation of these species using standard gradient elution or isocratic elution is very difficult. Using an optimized, shallow gradient RP-HPLC met...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Yokota H,Saito H,Masuoka K,Kaniwa H,Shibanuma T

更新日期：2000-12-15 00:00:00

abstract：:The voltammetric behaviour of Enrofloxacin (I), Sparfloxacin (II) and Fleroxacin (III) was studied using direct current (DCt), differential pulse (DPP) and alternating current (ACt). All the drugs manifest cathodic waves in Britton Robinson buffer over the pH range of 4.0-11.98. The waves were characterized as being i...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rizk M,Belal F,Ibrahim F,Ahmed S,El-Enany NM

更新日期：2000-12-15 00:00:00

abstract：:Artesunate is the most widely used of the artemisinin derivatives. These drugs are being used increasingly throughout the tropical world, and are an essential component of the treatment of multi-drug resistant malaria. The recent and widespread appearance of counterfeit artesunate tablets in several countries in South...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Green MD,Mount DL,Wirtz RA,White NJ

更新日期：2000-12-01 00:00:00

abstract：:A selective and sensitive isocratic high-performance liquid chromatographic (HPLC) method was developed for the quantitative analysis of low concentrations of fluoxetine (FLX) in human plasma, with ultra-violet detection at 226 nm. A reversed-phase column, LiChrospher 60 RP-Select B (125 x 3 mm i.d., 5 microm) (Merck)...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Maya MT,Domingos CR,Guerreiro MT,Morais JA

更新日期：2000-11-01 00:00:00

abstract：:This paper presents a facile and selective method for the determination of the pharmaceutical compound nafronyl using heavy atom induced room temperature phosphorimetry (HAI-RTP) as analytical technique. The determination was performed in potassium iodide 1.6 M and sodium sulphite 0.002 M at a measurement temperature ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Cruces-Blanco C,Seguro Carretero A,Fernández Sánchez JF,Fernández Gutiérrez A

更新日期：2000-10-01 00:00:00

abstract：:CGS 26214 is a racemic compound having cholesterol-lowering activity in rats, dogs, and monkeys. This compound has two equipotent chiral components CGS 28934(-) and CGS 28935(+). An analytical challenge was to develop a sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the analysis of the ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Majumdar TK,Martin LL,Melamed D,Tse FL

更新日期：2000-09-01 00:00:00